![]() Periodic acid-Schiff (PAS) kit was purchased from Shanghai Sun Biotechnology Co., Ltd (Shanghai, China). Masson’s trichrome stain kit (HT15), Cell Counting Kit-8, and H 2O 2 solution () were obtained from Sigma-Aldrich (St Louis, MO, USA). We also examined the effects of hydrogen gas exposure on the activation of ERK1/2 and NF-κB-dependent inflammatory responses induced by hydrogen peroxide (H 2O 2) in human bronchial epithelial cells.Īll cell culture reagents were purchased from Gibco (Carlsbad, CA, USA). In the present study, we employed mouse model of COPD with CS exposures, and evaluated the effects of hydrogen gas on the development of COPD. These findings strongly indicate that H 2 treatment may be beneficial in treating COPD. Furthermore, higher concentrations of H 2 showed better outcome compared to lower concentrations of H 2 ( 24). ![]() H 2 inhalation attenuated lung inflammatory responses in CS-induced COPD in rats. reported that H 2 saturated water reduced airway remodelling and airway mucus hypersecretion by reducing NF-κB activation in asthmatic mice ( 23). ![]() H 2-rich saline also attenuates cigarette smoke (CS)-induced airway mucus production in rats ( 22). Furthermore, inhalation of H 2 or ingestion of H 2-rich water can effectively prevent acute lung injury in mice induced by oxygen poisoning, ventilator injury, ischaemia-reperfusion injury, or sepsis ( 20, 21). Clinical trials have confirmed that H 2 can be used to treat cerebral ischaemia, uraemia ( 13), diabetes ( 14), metabolic syndrome ( 15), erythema skin disease, pressure ulcers, malignancies, rheumatism, arthritis ( 16, 17), side-effects associated with tumor radiotherapy and chemotherapy ( 18), mitochondrial muscle disease, and Parkinson's disease ( 19). OH) directly, which may contribute to its anti-inflammatory and anti-oxidative lesions.Hydrogen gas is able to react with hydroxyl radicals ( It has been shown that H 2, a physiologically regulatory gas molecule, exerts anti-inflammatory ( 9), antioxidant ( 10), anti-apoptotic ( 11) and signalling-regulating effects ( 12). Therefore, it is necessary to develop new and effective medicine to prevent or treat COPD with fewer side-effects. However, there is no known pharmaceutical therapy for COPD that is able to reverse lung function decline ( 5- 8). Currently, COPD drug therapy is used to relieve symptoms, improve athletic ability and health status as well as reduce the frequency and severity of exacerbation. Cigarette smokers have a higher prevalence of respiratory symptoms, lung function abnormalities, and COPD mortality rate than non-smokers ( 4). A strong relationship has been identified between continuous tobacco use, oxidative stress, and exacerbation of COPD symptoms ( 3). Cigarette smoking is the most common risk factor for COPD. Accepted for publication May 10, 2018.Ĭhronic obstructive pulmonary disease (COPD) is the fourth-leading cause of death in the world ( 1), imposing a substantial economic and social burden ( 2). Keywords: Hydrogen gas chronic obstructive pulmonary disease (COPD) cigarette smoke oxidative stress, inflammation These changes were reduced by H 2 treatment.Ĭonclusions: These findings demonstrated that H 2 inhalation could inhibit CS-induced COPD development in mice, which is associated with reduced ERK1/2 and NF-κB-dependent inflammatory responses. In 16HBE airway cells, H 2O 2 increased IL-6 and IL-8 secretion in conjunction with ERK1/2 and NF-κB activation. Results: H 2 ameliorated CS-induced lung function decline, emphysema, inflammatory cell infiltration, small-airway remodelling, goblet-cell hyperplasia in tracheal epithelium and activated ERK1/2 and NF-κB in mouse lung. The levels of phosphorylated ERK1/2 and nucleic NF-κB in lungs and 16HBE cells were determined. The IL-6 and IL-8 levels in cell culture medium were measured. H 2O 2 was used to treat 16HBE cells with or without H 2 pretreatment. Furthermore, 16HBE cells were used to evaluate the effects of H 2 on signaling change caused by hydrogen peroxide (H 2O 2). The lung tissue was subjected to H&E or PAS or Masson’s trichrome stain. The inflammatory cells were counted and the levels of IL-6 and KC in BALF were assayed with ELISA. ![]() Lung functions were assessed by using Buxco lung function measurement system. The H 2 intervention was administered by atomisation inhalation. Methods: A COPD mouse model was established in male C57BL mice by cigarette smoke (CS) exposure. This study aimed to evaluate the beneficial effects of H 2 inhalation on COPD development in mice. Hydrogen (H 2) has been shown to be anti-oxidative and anti-inflammatory.
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